Coding

Part:BBa_K5374022:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-24)


COMP-EGFP (Cartilage Oligomeric Matrix Protein). A protein that binds and stabilizes collagen struct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1306
    Illegal PstI site found at 133
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 133
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1949


Design Notes

Sequence was optimized to maintain COMP’s collagen-binding properties while preserving EGFP’s fluorescence for effective functional analysis.


Source

Derived from COMP gene, with EGFP from BBa_K1911005.